+66-7428-8797 mbbpsuofficial

Prof. Dr. Amornrat Phongdara


Gender: Female
Nationality: Thai  
Religion: Buddhism
Present Status: Professor/ Principle investigator 
Address: Center for Genomics and Bioinformatics Research.
                Faculty of Science, Prince of Songkla University, 
                Hat-Yai, Songkhla 90112, Thailand.
Telephone: (+66)-74-288785
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.This email address is being protected from spambots. You need JavaScript enabled to view it.

Research of Interest: Functional genomic studies of animal and plant genes, Molecular biology of Fortilin in shrimp immune                                                                                                    

Education Background

1974-1978 B.Sc., Hons (Medical Technology), Faculty of Medical Technology, Mahidol University, Bangkok, Thailand
1978-1980 M.Sc. (Biochemistry), Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
1982-1987 Doctor of Engineering (Biotechnology), Department of Biotechnology, Faculty of Engineering, Osaka University, Osaka, Japan.
1989-1990 Postdoctoral fellow, Alexander Von Humboldt Stiftung, Institut fur Mikrobiologie, Heinrich-Heine Universitat Dusseldorf, Dusseldorf, Germany. Project title: “Cloning of Acid Phosphatase Gene from Yeast Hansenula polymorpha”
2002 Certificate in Bioinformatics (University of Oxford)
2003 Certificate in Molecular Ecology (Lincoln University, New Zealand)


Scientific Experiences

Yeast Genetic, genomic and cDNA cloning, DNA sequencing, site-directed mutagenesis, temperature sensitive mutants selection, Southern and Northern hybridization, Molecular markers and DNA fingerprinting, Yeast two hybrid system.

Current Researches Topics

  • Functional genomic studies of animal and plant genes.
  • Molecular biology of Fortilin in shrimp immune.
  • The application of recombinant protein to aquaculture.
  • DNA markers for species identification and population studies of marine organism.
  • Data mining of animal and plant genomes.


  • Ruttajorn, K., Nakkaew, A., Panrat, T., Vanichanon, A., Phongdara, A. (2016). Isolation, expression analysis and characterization of EgNDL, a NDR-like protein in oil palm (Elaeis guineensis Jacq.). Songklanakarin Journal of Science and Technology, 38(3), 281-287.
  • Tantikitti, C., Chookird, D., Phongdara, A. (2016). Effects of fishmeal quality on growth performance, protein digestibility and trypsin gene expression in pacific white shrimp (Litopenaeus vannamei). Songklanakarin Journal of Science and Technology, 38(1), 73-82.
  • Puwaphut, R., Nakkaew, A., Phongdara, A. (2016). Diversity among 3 cultivars (RRIM600, RRIT251, and PB350) of hevea brasiliensis and secondary metabolite production. Songklanakarin Journal of Science and Technology, 38(1), 15-22.
  • Watthanasurorot, A.,Saelee, N.,Phongdara, A.,Roytrakul, S.,Jiravanichpaisal, P.,Söderhäll, K.,Söderhäll, I. (2015). Retraction: Astakine 2—the Dark Knight Linking Melatonin to Circadian Regulation in Crustaceans [PLoS Genet, (2015)]. PLoS Genetics, 11(4), e1005222, 1p.
  • Sinthujaroen, P., Tonganunt-Srithaworn, M., Eurwilaichitr, L.,Phongdara, A. (2015). Protection of Litopenaeus vannamei against the white spot syndrome virus using recombinant Pm-fortilin expressed in Pichia pastoris. Aquaculture, 435, 450-457.
  • Sinthujaroen, P., Wanachottrakul, N., Pinkaew, D., Petersen, J. R., Phongdara, A., Sheffield-Moore, M., et al. (2014). Elevation of serum fortilin levels is specific for apoptosis and signifies cell death in vivo. BBA Clin, 2, 103-111.
  • Lertwimol, T., Sangsuriya, P., Phiwsaiya, K., Senapin, S., Phongdara, A., Boonchird, C., et al. (2014). Two new anti-apoptotic proteins of white spot syndrome virus that bind to an effector caspase (PmCasp) of the giant tiger shrimp Penaeus (Penaeus) monodon. Fish Shellfish Immunol, 38(1), 1-6.
  • Kongton, K., McCallb, K., Phongdara, A. (2014). Identification of gamma-interferon-inducible lysosomal thiol reductase (GILT) homologues in the fruit fly Drosophila melanogaster. Developmental & Comparative Immunology. Available online 31 January 2014.
  • Nakkaew, A.,Phongdara, A.,Eksomtramage, T.,Chotigeat, W. (2014). Over expression and application of the beta-carboxyltransferase (EgaccD) gene in oil palm (Elaeis guineensis Jacq). Songklanakarin Journal of Science and Technology, 36(1), 57-64.
  • Kowasupat, C., Panijpan, B., Laosinchai, P., Ruenwongsa, P., Phongdara, A., Wanna, W., W., Saengchan, S., Phiwsaiya, K. (2014). Biodiversity of the Betta smaragdina (Teleostei: Perciformes) in the northeast region of Thailand as determined by mitochondrial COI and nuclear ITS1 gene sequences. Meta Gene 2(0): 83-95.
  • Panijpan, B., Kowasupat, C., Laosinchai, P., Ruenwongsa, P., Phongdara, A., Senapin, S., Wanna, W., Phiwsaiya, K., Kühne, J.,Fasquel, F. (2014). Southeast Asian mouth-brooding Betta fighting fish (Teleostei: Perciformes) species and their phylogenetic relationships based on mitochondrial COI and nuclear ITS1 DNA sequences and analyses. Meta Gene, 2, 862-879.
  • Saelee, N., Noonin, C., Nupan, B., Junkunlo, K., Phongdara, A., Lin, X., Soderhall, K., Soderhall, I. (2013). Beta-thymosins and hemocyte homeostasis in a crustacean. PLoS One 8(4): e60974
  • Rujananon, R., Prasertsan, P., Phongdara, A. (2013). Biosynthesis of 1,3-propanediol from recombinant E. coli by optimization process using pure and crude glycerol as a sole carbon source under two-phase fermentation system. World J Microbiol Biotechnol.
  • Meemak, P., Phongdara, A., Chotigeat, W., Tammi, M.T. (2013). Computational identification of Penaeus monodon microRNA genes and their targets. Songklanakarin Journal of Science and Technology, 35(2), 143-148.
  • Watthanasurorot, A., Saelee, N., Phongdara, A, Roytrakul, S., Jiravanichpaisal, P., Söderhäll, K., Söderhäll, I. (2013). Astakine 2—the Dark Knight Linking Melatonin to Circadian Regulation in Crustaceans. PLoS Genet 9(3): e1003361. doi:10.1371/journal.pgen.1003361.
  • Hualkasin, W., Thongin, W., Petsean, K., Phongdara, A., Nakkaew, A. (2013). Molecular cloning and characterization of the late embryogenesis abundant group 4 (EgLEA4) gene from oil palm (Elaeis guineensis Jacq). Songklanakarin Journal of Science and Technology, 35(3), 275-285.
  • Nakkaew, A., Thitichai, N., Nualkaew, S., Chotigeat, W., Phongdara, A. (2013). Cloning, characterization and overexpression of a 14-3-3 ω protein from oil palm (Elaeis guineensis). Plant Breeding, 132(6), 701-710.
  • Kanchanapoom, K., Nakkaew, A., Kanchanapoom, K., Phongdara, A.* (2012). Efficient biolistic transformation and regeneration capacity of an EgTCTP transgene in protocorm-like bodies of Phalaenopsis orchid Notulae Botanicae Horti. Agrobotanici Cluj-Napoca, 40 (1), 58-64.
  • Phusantisampan, T., Sangkhathat, S., Phongdara, A., Chiengkriwate, P., Patrapinyokul, S., Mahasirimongkol, S. (2012). Association of genetic polymorphisms in the RET-protooncogene and NRG1 with Hirschsprung disease in Thai patients. Journal of Human Genetics, 57 (5), 286-293.
  • Wanna, W., Thipwong, J., Mahakaew, W., Phongdara, A. (2012). Identification and expression analysis of two splice variants of the 14-3-3 epsilon from Litopenaeus Vannamei during WSSV infections. Molecular Biology Reports, 39 (5), 5487-5493.
  • Panrat, T., Sinthujaroen, P., Nupan, B., Wanna, W., Tammi, MT., Phongdara, A.* (2012). Characterization of a novel binding protein for Fortilin/TCTP - component of a defense mechanism against viral infection in Penaeus monodon . PLoS ONE, 7(3), art. no. e33291.
  • Phongdara, A.*, Nakkaew, A., Nualkaew, S. (2012). Isolation of the detoxification enzyme EgP450 from an oil palm EST library (2012). Pharmaceutical Biology, 50(1), 120-127.
  • Khimmakthong, U., Deachamag, P., Phongdara, A., Chotigeat, W. (2011). Stimulating the immune response of Litopenaeus vannamei using the phagocytosis activating protein (PAP) gene. Fish and Shellfish Immunology, 31(3), 415-422.
  • Busayarat, N., Senapin, S., Tonganunt, M., Phiwsaiya, K., Meemetta, W., Unajak, S., Jitrapakdee, S., Lo, C.-F., Phongdara, A.* (2011). Shrimp laminin receptor binds with capsid proteins of two additional shrimp RNA viruses YHV and IMNV. Fish and Shellfish Immunology, 31 (1), 66-72.
  • Saelee, N., Tonganunt-Srithaworn, M., Wanna, W., Phongdara, A.* (2011). Receptor for Activated C Kinase-1 protein from Penaeus monodon (Pm-RACK1) participates in the shrimp antioxidant response. International Journal of Biological Macromolecules, 49(1), 32-36.
  • Kongton, K., Phongdara, A.*, Tonganunt-Srithaworn., M., Wanna, W. (2011). Molecular cloning and expression analysis of the interferonc-γ inducible lysosomal thiol reductase gene from the shrimp Penaeus monodon. Molecular Biology Reports, 38 (5), 3463-3470.
  • Laoong-u-thai, Y., Zhao, B., Phongdara, A., Yang, J. (2011). Molecular Characterizations of a Novel Putative DNA-Binding Protein LvDBP23 in Marine Shrimp L. vannamei Tissues and Molting Stages. PLoS ONE, 6(5), art. no. e19959.
  • Rujananon, R., Prasertsan, P., Phongdara, A., Panrat, T., Sun, J., Rappert, S., Zeng, AP. (2011). Construction of recombinant E.coli expressing fusion protein to produce 1,3-propanediol. World Academy of Science, Engineering and Technology, 76, 507-513.
  • Nupan, B., Phongdara, A., Saengsakda, M., Leu, JH., Lo, CF. (2011). Shrimp Pm-fortilin inhibits the expression of early and late genes of white spot syndrome virus (WSSV) in an insect cell model. Developmental and Comparative Immunology, 35 (4), 469-475.
  • Youtong, W., Deachamag, P., Phongdara, A., Chotigeat, W. (2011). WSSV: VP26 binding protein and its biological activity. Fish and Shellfish Immunology, 30(1), 77-83.
  • Kanchanapoom, K., Phongdara, A., Kanchanapoom, K. (2010). The effect of chitosan on the organogenesis of oil palm embryo-derived callus. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38, 213-217.
  • Chotigeat, W., Duangchu, S., Phongdara, A. (2010). CDNA library from the latex of Hevea brasiliensis. Songklanakarin Journal of Science and Technology, 32(6), 555-559.
  • Nakkaew, A., Chotigea, W., Phongdara, A. (2010). Molecular cloning and expression of EgTCTP, encoding a calcium binding protein, enhances the growth of callus in oil palm (elaeis guineensis, jacq). Songklanakarin Journal of Science and Technology, 32(6), 561-569.
  • Wonglapsuwan, M., Phongdara, A., Chotigeat, W.(2009). Dynamic changes of gene expression during vitellogenic stages of the white shrimp: Fenneropenaeus merguiensis de man. Aqua. Res. 40, 633-643. (IF 1.067)
  • Chotigeat, W.,Duangchu, S.,Wititsuwannakun, W., Phongdara, A. (2009). Cloning and characterization of pectate lyase from Hevea brasiliensis. Plant Physiol. 47, 243-247. (IF 1.669)
    Koide, Y., Kiyota, T., Tonganunt, M., Pinkaew, D., Liu, Z., Kato, Y., Hutadilok-Towatana, N., Phongdara, A., and Fujise, Ken. (2009). Embryonic lethality of fortilin-null mutant mice by BMP-pathway overactivation. Biochimica et Biophysica Acta 1790, 326-338.
  • Tonganunt, M., Saelee, N., Chotigeat, W. and Phongdara, A.* (2009). Identification of a receptor for activated protein kinase C1 (Pm-RACK1), a cellular gene product from black tiger shrimp (Penaeus monodon) interacts with a protein, VP9 from the white spot syndrome virus. J. Fish & Shellfish Immunol 26, 509-514 doi:10.1016/j.fsi.2009.02.002. (IF 3.16)
  • Laoong-u-thai, Y., Zhao, B., Phongdara, A.*, Ako, H. and Yang, J. (2009). Identifications of SUMO-1 cDNA and Its Expression Patterns in Pacific White Shrimp Litopeanaeus vannamei. Int. J. Biol. Sci. 5(2), 205-214. (IF 3.24)
  • Pinkaew, D., Cho, S.G., Hui, D.Y., Wiktorowicz, J.E., Hutadilok-Towatana, N., Mahabusarakam, W., Tonganunt, M., Stafford, L.J., Phongdara, A., Liu, M. and Fujise, K. (2009). Morelloflavone blocks injury-induced neointimal formation by inhibiting vascular smooth muscle cell migration. Biochimica et Biophysica Acta 1790, 31–39. doi:10.1016/j.bbagen.2008.09.006
  • Tonganunt, M., Nupan, B., Suklour, S., Saengsakda, M., Senapin, S., Chotigeat, W. and Phongdara, A.* (2008). The role of Pm-fortilin in protecting shrimp from White Spot Syndrome Virus (WSSV) infection. J. Fish & Shellfish Immunol 25, 633-637. doi:10.1016/j.fsi.2008.08.06. (Nov.) (IF 3.16)
  • Nakkaew, A., Chotigeat, W., Eksomtramage, and Phongdara, A*. (2008). Cloning and expression of a plastid-encoded subunit, beta-carboxyltransferase gene (accD) and a nuclear-encoded subunit, biotin carboxylase of acetyl-CoA carboxylase from oil palm (Elaeis guineensis Jacq.). Plant Science 175, 497-504. doi:101016/j.plantsci.2008.05.023.
  • Tonganunt, M., Wongmanee, K., Sartthai, S., Chotigeat, W. and Phongdara, A. (2008). Crustin protein Amk1 from black tiger shrimp (Penaeus monodon) inhibits Vibrio harveyi and Staphylococcus aureus. Songklanakarin J. Sci.Technol. 30(3), 291-296. (May-Jun.)
  • Hualkasin, W., Tongchuai, W., Chotigeat, W. and Phongdara, A.* (2008). Phylogeography of ivory shell (Babylonia areolata) in the gulf of Thailand revealed by COI gene structure and differentiation of shell color by ITS1 DNA. Songklanakarin J. Sci. Technol. 30 (2), 141-146. (Mar.-Apr.)
  • Kanchanapoom, K., Nakkaew, A., Kanchanapoom, K. and Phongdara, A.* (2008). Determination of the most efficient target tissue and helium pressure for biolistic transformation of oil palm (Elaeis guineensis Jacq). Songklanakarin J. Sci. Technol. 30 (2), 153-157. (Mar.-Apr.)
  • Sangket, U., Phongdara, A., Chotigeat, W., Nathan, D., Kim, W.Y., Bhak, J., Ngamphiw, C., Tongsima, S., Khan, A.M., Lin, H. and Tan, T.W. (2008). Automatic synchronization and distribution of biological databases and software over low-bandwidth networks among developing countries. Bioinformatics 24, 299-301. doi:10.1093/bioinformatics/btm570 (IF = 5.039)
  • Ruensirikul, J., Angsupanich, S. and Phongdara, A.* (2007). Abundance and diversity of amphipod crustaceans in the upper songkhla lagoon. Songklanakarin J. Sci. Technol. 29, 1225-1249.
  • Graidist, P., Yazawa, M., Tonganunt, M., Nakatomi, A., Lin, C.C.J., Chang, J.Y., Phongdara, A., and Fujise, K. (2007). Fortilin binds Ca2+ and blocks Ca2+-dependent apoptosis in vivo. Biochem. J. 408, 181-191. doi:10.1042/BJ20070679 (17 Aug. 2007)
  • Chotigeat, W., Dechamag, P. and Phongdara, A. (2007). Identification of a protein binding to the phagocytosis activating protein (PAP) in immunized black tiger shrimp. Aquaculture 271, 112-120. (IF 2.08)
  • Phongdara, A.*, Laoong-u-thai, Y. and Wanna, W. (2007). Cloning of eIF5A from Shrimp Penaeus monodon, a Highly expressed protein involved in the survival of WSSV infected shrimp. Aquaculture 265, 16-26. (1 May 2007) (IF 2.08)
  • Senapin, S. and Phongdara, A.* (2006). Binding of shrimp cellular proteins to Taura syndrome viral capsid proteins VP1, VP2 and VP3. Virus Research 122, 69-77. (Dec 2006) (IF 2.56)
  • Tassanakajon, A. , Klinbunga, S. , Paunglarp, N. , Rimphanitchayakit, V., Udomkit, A., Jitrapakdee, S. , Sritunyalucksana, K., Phongdara, A., Pongsomboon, S., Supungul, P., Tang, S., Kuphanumart, K., Pichyangkura, R. and Lursinsap, C. (2006). Penaeus monodon gene discovery project: The generation of an EST collection and establishment of a database. Gene 384, 104-112. (15 Dec 2006) (IF 2.694)
  • Loongyai, W., Phongdara, A. and Chotigeat, W. (2006). Cloning and expression of a TCTP homolog from the ovaries of banana prawn. Marine Biology 150, No 1432-1793. (Jan 2007) (IF 1.754)
  • Deachamg, P., Intaraphad, U., Phongdara, A. and Chotigeat, W. (2006). Expression of phagocytosis activating protein (PAP) gene in immunized black tiger shrimp. Aquaculture 255/1-4, 165-172. (May 2006) (IF 2.08)
  • Suttapreyasri, S., Koontongkaew, S., Phongdara, A. and Leggat, U. (2006). Expression of bone morphogenetic proteins in normal human intramembranous and endochondral bone. J. Oral Maxillofacial Surgery 35, 444 – 452. (online 2 March, 2006)
  • Graidist, P., Fujise, K., Sritunyalucksana, K. and Phongdara, A.* (2006). Establishing a role for shrimp fortilin/TCTP in apoptosis. Aquaculture 255/1-4, 157-164. (online 13 February, 2006) (IF 2.08)
  • Wanna, W., Phongdara, A.* and Chotigeat, W. (2006). Sequence variation of the first ribosomal internal transcribed spacer of Penaeus merguiensis in Thailand. Exp. Mol. Biol. Ecol. 331/1, 64-73. (online 13 December, 2005)(IF 1.66)
  • Phongdara, A.*, Wanna, W. and Chotigeat, W. (2006). Molecular cloning and expression of caspase-3 from white shrimp, Penaeus merguiensis. Aquaculture 252/2-4, 114-120. (online 25 Aug. 2005). (IF 2.08)
  • Tonganunt, M., Chotigeat, W., Fujise, K. and Phongdara, A.* (2005). Identification of syntenin binding protein in the black tiger shrimp Penaeus monodon. J. Biotech. 120, 135-145. (IF 2.687)
  • Graidist, P., Phongdara, A. and Fujise, K. (2004). The independent anti-apoptotic activities of fortilin and its protein partner MCL1. J. Biol. Chem. 279, 39, 40868-40875. (IF 5.854)
  • Wanna, W., Rolland, J.L., Bonhomme, F. and Phongdara, A.* (2004) Population genetic structure of Penaeus merguiensis in Thailand based on nuclear DNA variation. J. Exp. Mol. Biol. Ecol. 311, 63-78. (IF 1.66)
  • Phongdara, A.*, Hualkasin, W., Wanna, W., Chanumpai, A. and Chotigeat, W. (2004) Species identification of white shrimps Litopenaeus vannamei and other Penaeus sp. by using PCR-RFLP of cytochrome oxidase subunit I (COI) gene. Songklanakarin J. Sci. Technol. 26, 4, 467-478.
  • Chotigeat, W., Tongsupa, S., Supamataya, K. and Phongdara, A.* (2004) Effect of fucoidan on disease resistance of black tiger shrimp. Aquaculture 233/1-4, 23-30. (IF 2.08)
  • Bangrak, P., Graidist, P., Chotigeat, W. and Phongdara, A.* (2004) Molecular cloning and expression of a mammalian homologue of a translationally controlled tumor protein (TCTP) gene from Penaeus monodon shrimp. J. Biotech. 103/3, 219-226. (IF 2.687)
  • Hualkasin, W., Sirimontaporn, P., Chotigeat, W., Querci, J.and Phongdara, A.* (2003) Molecular phylogenetic analysis of white prawns species and the existence of two clades in Penaeus merguiensis. J. Exp. Mol. Biol. Ecol. 296/1, 1-11. (IF 1.66)
  • Sretrirutchai, S., Silapapojakul, K., Palittapongarnpim, P., Phongdara, A. and Vuddhakul, V. (2002) Tuberculosis in Thai prisons: magnitude, transmission and drug susceptibility. Int. J. Tuberc. Lung Dis. 6, 208-214. (IF 2.24)
  • Bangrak, P., Phongdara, A.*, Graidist, P., Chotigeat, W. and Supamattaya, K. (2002) A syntenin-like protein with PDZ domains produced by black tiger shrimp(Penaeus monodon) in response to white spot syndrome virus infection. Dis. Aquat. Org. 49, 19-25. (IF 1.598)
  • Sripo, T., Phongdara, A.*, Wanapu, C., and Caplan, A.B. (2002) Characterization and expression of aldehyde dehydrogenase gene from Halomonas salina AS11. J. Biotech 95/2, 171-179. (IF 2.687)
  • Chotigeat, W., Buatong, W., and Phongdara, A. (2001). Detection of dengue virus by reverse transcriptase-polymerase chain reaction (RT-PCR) in serum of patients. Songklanakarin J. Sci. Technol. 23, 376-381.
  • Chotigeat, W., Chayanunnukul, W. and Phongdara, A. (2000) Expression of a mammalian a 2, 6 sialyltransferase in Pichia pastoris. J. Biotech.81, 65 - 71. (IF 2.687)