.

+66-7428-8797 mbb.sci.psu@gmail.com

Assoc. Prof. Dr. Wilaiwan Chotigeat

Gender: Female
Nationality: Thai
Religion: Buddhism
Present Status: Associate Professor / Principle investigator
Address: Faculty of Science, Prince of Songkla University, Hat-Yai, Songkla 90112, Thailand.
Telephone: +66-74-288784,
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. / This email address is being protected from spambots. You need JavaScript enabled to view it.
Research of Interest: Mechanism of ovarian maturation in shrimp, Molecular and biology of immunne response in shrimp

Education backgrounds

Ph.D.   (Biotechnology) from The university of New South Wales
M.Sc.   (Biochemistry) from Mahidol University
B.Sc.    (Medical Technology 1st honour) from Mahidol University

Research and experiences

Mechanism of ovarian maturation in shrimp, molecular and biology of immune response in shrimp, viral disease in shrimp, study of glycosylation and tissue engineering of proteins, functional genomic in shrimp and rubber tree

Patents and Junior Patents

  • สิทธิบัตร กรรมวิธีการผลิตโปรตีนที่จับกับ VP26 ของไวรัสตัวแดงดวงขาว(White Spot Syndrome Virus Binding Protein, BPVP26) เลขที่คำขอ 1001001995
    ผู้ประดิษฐ์ นางสาววิไลวรรณ โชติเกียรติ นางอมรรัตน์ พงศ์ดารา นางสาวปัญชลิกา เดชะมาก นายวีรยุทธ อยู่ทอง
  • สิทธิบัตร โปรตีนฟอติลินและการใช้ เลขที่คำขอ 0701002088
    ผู้ประดิษฐ์ นางอมรรัตน์ พงศ์ดารา และนางสาววิไลวรรณ โชติเกียรติ
  • อนุสิทธิบัตร กรรมวิธีการตรวจวัดการแสดงออกของยีนเบต้าคาร์บอกซิลทรานสเฟอเรส (beta-carboxyltransferase) หน่วยย่อย D (accD) ในปาล์มน้ำมัน เลขที่คำขอ 6088
    ผู้ประดิษฐ์ นางสาววิไลวรรณ โชติเกียรติ นางอมรรัตน์ พงศ์ดารา นางสาวอลิษา หนักแก้ว และนายธีระ เอกสมทราเมษฐ์
  • อนุสิทธิบัตร ส่วนผสมของเรซินประยุกต์กลาสไอโอโนเมอร์ซีเมนต์และกรรมวิธีการผลิตส่วนผสมดังกล่าว เลขที่คำขอ 1001001095
    ผู้ประดิษฐ์ รศ. ดร. อุรีพร เล็กกัต, รศ. ดร. วิไลวรรณ โชติเกียรติ และนางสาวณัฐพร วนโชติตระกูล

Publications

  • Navakanitworakul, R., Hung, W.-T., Gunewardena, S., Davis, J.S., Chotigeat, W., Christenson, L.K.2016. Characterization and small RNA content of extracellular vesicles in follicular fluid of developing bovine antral follicles.Scientific Reports, 6, 25486.
  • Waiyapoka, T., Deachamag, P., Chotigeat, W., Bunsanong, N., Kanatharana, P., Thavarungkul, P., Loyprasert-Thananimit, S.2015. Application of a Label-Free Immunosensor for White Spot Syndrome Virus (WSSV) in Shrimp Cultivation Water. Applied Biochemistry and Biotechnology,177 (4), pp. 821-830.
  • Maikaeo, L., Chotigeat, W., Mahabusarakam, W.2015. Emilia sonchifolia extract activity against white spot syndrome virus and yellow head virus in shrimp cell cultures.Diseases of Aquatic Organisms, 115 (2), pp. 157-164.
  • Anuchan, S., Deachamag, P., Siammai, N., Phongpaichit, S., & Chotigeat, W. (2015). Antimicrobial Activity of Engineered Shrimp Ovarian Peritrophin Fragments from Fenneropenaeus merguiensis.Protein Pept Lett, 22(1), 73-80.
  • Palasin, K., Makkapan, W., Thongnoi, T., Chotigeat, W. (2014). Stimulation of ovarian development in white shrimp, Fenneropenaeus merguiensis De Man, with a recombinant ribosomal protein L10a.Aquaculture, 432, pp. 38-45.
  • Nakkaew, A., Phongdara, A., Eksomtramage, T., Chotigeat, W. (2014). Over expression and application of the β-carboxyltransferase ZEgaccD) gene in oil palm (Elaeis guineensis Jacq.Songklanakarin Journal of Science and Technology, 36 (1), pp. 57-64.
  • Pornpunyapat, J., Chotigeat, W., Chetpattananondh, P.2014. Bioethanol production from pineapple peel juice usingsaccharomyces cerevisiae.Advanced Materials Research,875-877, pp. 242-245.
  • Engsontia, P., Sangket, U., Chotigeat, W., & Satasook, C. (2014). Molecular evolution of the odorant and gustatory receptor genes in lepidopteran insects: implications for their adaptation and speciation. J Mol Evol, 79(1-2), 21-39.
  • Kedjarune-Leggat, U., Supaprutsakul, C., & Chotigeat, W. (2014). Ultrasound treatment increases transfection efficiency of low molecular weight chitosan in fibroblasts but not in KB cells.PLoS One, 9(3), e92076.
  • Kitpipit, T., Chotigeat, W., Linacre, A., & Thanakiatkrai, P. (2014). Forensic animal DNA analysis using economical two-step direct PCR.Forensic Sci Med Pathol, 10(1), 29-38.
  • Loyprasert-Thananimit, S., Saleedang, A., Deachamag, P., Waiyapoka, T., Neulplub, M., & Chotigeat, W. (2014). Development of an immuno-based colorimetric assay for white spot syndrome virus. Biotechnol Appl Biochem, 61(3), 357-362.
  • Makkapan, W., Yoshizaki, G., Tashiro, M., & Chotigeat, W. (2014). Expression profile of ribosomal protein L10a throughout gonadal development in rainbow trout (Oncorhynchus mykiss).Fish Physiol Biochem, 40(4), 1069-1081.
  • Wanachottrakul, N.,Chotigeat, W., & Kedjarune-Leggat, U. (2014). Effect of novel chitosan-fluoroaluminosilicate resin modified glass ionomer cement supplemented with translationally controlled tumor protein on pulp cells.J Mater Sci Mater Med, 25(4), 1077-1085.
  • Kitpipit, T., Thanakiatkrai, P., Chotigeat, W. (2013). Direct PCR-FINS: Wildlife species identification without DNA extraction.Forensic Science International: Genetics Supplement Series, 4 (1), pp. e364-e365.
  • Nakkaew, A., Thitichai, N., Nualkaew, S., Chotigeat, W.,Phongdara, A.2013. Cloning, characterization and overexpression of a 14-3-3 ω protein from oil palm (Elaeis guineensis).Plant Breeding,132 (6), pp. 701-710.
  • Khimmakthong, U., Kongmee, P., Dechamag, P., Leggat, U., Chotigeat, W*.(2013) Activation of an immune response in Litopenaeus vannamei by oral immunization with Phagocytosis Activating Protein (PAP) DNA.Fish & shellfish Immunology, 34(3), 929-38. (IF 3.322)
  • Navakanitworakul, R., Deachamag, P., Wonglapsuwan, M, Chotigeat, W.* (2012) Roles of Ribosomal protein S3a in ovarian development of Fenneropenaeus merquiensis (de man) Aquaculture 338-341, 208-215 (IF 2.10)
  • Loyprasert-Thananimit, S., Saleedang, A., Kanatharana, P., Thavarungkul, P., Chotigeat, W. (2012) Production of a polyclonal antibody to the VP26 nucleocapsid protein of white spot syndrome virus (wssv) and its use as a biosensor. Frontiers of Chemical Science and Engineering 6(2), 216-223.
  • Loyprasert-Thananimit, S., Kuasuwan, P., Nittayaboon, K., Chotigeat, W. and Chandeying, V. (2012) Validity evaluation of in-house preparation kit, vaginal pH paper test combined amine tube test, for the simple diagnosis of bacterial vaginosis. J Med Assoc Thai 95(6), 747-51. IF 0.562
  • Khimmakthong, U., Deachamag, P., Phongdara, A., Chotigeat, W. (2011). Stimulating the immune response of Litopenaeus vannamei using the phagocytosis activating protein (PAP) gene.Fish and Shellfish Immunology, 31 (3), pp. 415-422.
  • Wanachottrakul, N., Chotigeat, W., Kedjarune-Leggat, U. (2011). Translationally Controlled Tumor Protein against apoptosis from 2-hydroxy-ethyl methacrylate in human dental pulp cells.Journal of Materials Science: Materials in Medicine, 22 (6), pp. 1479-1487.
  • Bangrak, P., Chotigeat, W. (2011). Molecular cloning and biochemical characterization of a novel cystatin from Hevea rubber latex.Plant Physiology and Biochemistry,49 (3), pp. 244-250.
  • Youtong, W., Deachamag, P., Phongdara, A., Chotigeat, W. (2011). WSSV: VP26 binding protein and its biological activity.Fish and Shellfish Immunology, 30 (1), pp. 77-83.
  • Makkapan, W., Maikaeo, L., Miyazaki, T., Chotigeat, W.* (2011) Molecular mechanism of serotonin via methyl farnesoate in ovarian development of white shrimp; Fenneropenaeus merguiensis de Man. Aquaculture 321(1-2), 101-107 (IF 2.10)
  • Wonglapsuwan, M., Chotigeat, W*., Timmons A., McCall, K., (2011) Rpl10A regulates oogenesis progression in the banana prawn Ferneropenaeus merguiensisand Drosophila melanogaster. General and Comparative endocrinology. 173(2), 356-363. (IF 3.106)
  • Samanman, S. , Kanatharana, P. , Chotigeat, W., Deachamag, P., Thavarungkul, P. (2011) Highly sensitive capacitive biosensor for detecting white spot syndrome virus in shrimp pond water. Journal of Virological methods., 173(1), 75-84
  • Dawan, S., Kanatharana, P., Chotigeat, W., Jitsurong, S. and Thavarungkul, P. 2011 Surface plasmon resonance immunosensor for rapid and specific diagnosis of melioidosis antibody. Southeast Asian J Trop Med Public Health 42(5), 1168-1177 (IF 0.33)
  • Wonglapsuwan, M., Miyazaki, T., Loongyai, W., Chotigeat, W*. (2010) Characterization and Biological Activity of the Ribosomal Protein L10a of the White Shrimp: Fenneropenaeus merquiensis de man during vitellogenesis . Mar. Biotech.12, 230-240 (IF 2.554)
  • Wonglapsuwan, M., Phongdara, A., Chotigeat, W.* (2009) Dynamic Changes of Gene Expression during Vitellogenic Stages of the White Shrimp: Fenneropenaeus merguiensis de man. Aqua. Res. 40, 633-643 (IF 1.067)
  • Loongyai, W., Avarre,J-C., Martine, C., Lubzens, E. and Chotigeat, W.* (2007) Isolation and functional Characterization of a new Shrimp Ovarian Peritrophin (SOP) from the ovary of Fenneropenaeus merguiensis. Mar. Biotech. 9(5), 624-637. (IF 2.408)
  • Loongyai, W., Phongdara, A. and Chotigeat, W.* (2006). Cloning and expression of a TCTP homologue from the ovaries of Banana prawn. Mar Biol. 150, 455-462. IF 1.754
  • Youtong, W., Deachamag, P., Phongdara, A., Chotigeat, W.* (2011) WSSV: VP26 Binding Protein and its Biological Activity. Fish and shellfish Immunol. 30, 77-83. IF 3.160
  • Tonganunt, M., Saelee, N., Chotigeat, W., Phongdara., A. (2009) Identification of a receptor for activated protein kinase C1 (Pm-RACK1), a cellular gene product from black tiger shrimp (Penaeus monodon) interacts with a protein, VP9 from the white spot syndrome virus. Fish and shellfish Immunol. 26(3), 509-514. IF 3.160
  • Tonganunt, M., Nupan, B., Saensakda, M., Suklour, S., Wanna, W., Senapin, S.,Chotigeat, W., Phongdara, A. The role of Pm-fortilin in protecting shrimp from white spot syndrome virus (WSSV) infection. (2008) Fish and shellfish Immunology 25 (5), 633-637.
  • Chotigeat, W.*, Deachamag, P.and Phongdara, A. (2007) Identification of a protein bindinding to phagocytosis activating protein (PAP) in immunized black tiger shrimp. Aquaculture 271, 112-120. IF 2.081
  • Deachamag, P., Intaraphad, U., Phongdara, A. and Chotigeat, W.* (2006). Expression of a Phagocytosis Activating Protein (PAP) gene in immunized black tiger shrimp. Aquaculture 255/1-4, 165-172. IF 2..081
  • Chotigeat, W.*, Tongsupa, S., Supamataya, K. and Phongdara, A. (2004) Effect of fucoidan on disease resistance of black tiger shrimp. Aquaculture 233/1-4, 23-30. IF 2.081
  • Tonganunt, M., Nupan, B., Saensakda, M., Suklour, S., Wanna, W., Senapin, S.,Chotigeat, W., Phongdara, A. The role of Pm-fortilin in protecting shrimp from white spot syndrome virus (WSSV) infection. (2008) Fish and shelfish Immunology. (in press) (IF 3.160)
  • Phongdara, A., Wanna, W. and Chotigeat, W. (2005). Molecular cloning and expression of caspase-3 from white shrimp, Penaeus merguiensis. Aquaculture, 252/2-4, 114-120. IF 2.081
  • Tonganunt, M., Chotigeat, W., Fujise, K. and Phongdara, A.(2005). Identification of syntenin binding protein in the black tiger shrimp Penaeus monodon. J. Biotech. 120, 135-145. IF 2.687
  • Bangrak, P., Graidist, P., Chotigeat, W. and Phongdara, A.(2004) Molecular cloning and expression of a mammalian homologue of a translationally controlled tumor protein (TCTP) gene from Penaeus monodon shrimp. J. Biotech. 103/3, 219-226. IF 2.687
  • Bangrak, P., Graidist, P., Chotigeat, W., Supamattaya, K. and Phongdara, A.(2002). A Syntenin-like Protein with PDZ Domains Produced by Black Tiger Shrimp(Penaeus monodon) in Response to White Spot Syndrome Virus Infection. Dis. Aquat. Org. 49, 19-25. IF 2.6
  • Supaprutsakul, S., Chotigeat, W., Wanichpakorn, S., Kedjarune-Leggat., U. (2010) Transfection efficiency of depolymerized chitosan and epidermalgrowth factor conjugated to chitosan–DNA. J. Mater Sci: Mater Med, J. Material Science: Material Medicine, 173 (1), 75-84.
  • Chotigeat, W*., Duangchu, S., Wititsuwannakun,W., Phongdara, A. (2009) Cloning and Characterization of Pectate lyase from Hevea brasiliensis. Plant Physiol.47, 243-247. (IF 1.669)
  • Nakkaew, A., Chotigeat, W., Eksomtramage, T., Phongdara, A. Cloning and expression of a plastid-encoded subunit, beta-carboxyltransferase gene (accD) and a nuclear-encoded subunit, biotin carboxy of acetyl-CoA car boxylase from oil palm (Elaeis guineensis Jacq.) (2008) Plant Science, 175 (4), pp. 497-504. (IF 1.795)
  • Hualkasin, W., Tongchuai, W., Chotigeat, W., Phongdara, A. (2008) Phylogeography of Ivory shell (Babylonia areolata) in the gulf of Thailand revealed b COI gene structure and differentiation of shell color by ITS1 DNA. Songklanakarin J. Sci. Technol. 30(2), 141-146. (IF 0.118)
  • Musor, I., Chotigeat, W*., Phongdara, A. (2008) Cloning and Characterization of Protease Inhibitor from Latex of Rubber Tree (Hevea brasiliensis). KKu Research Journal, 8 (2), April-June, 12-17. (IF 0.022)
  • Visutthi, M., Jitsurong, S. and Chotigeat, W*. (2008) Production and Purification of Burkhoderia Pseudomallei BipD Protein. The Southeast Asian J Trop Med Public Heaith, 39 (1), 109-114. (IF 0.097)
  • Sangket, U., Phongdara, A., Chotigeat, W., Nathan, D., Kim, W.Y., Bhak, J., Ngamphiw, C., Tongsima, S., Khan, A.M., Lin, H. and Tan, T.W. (2007). Automatic synchronization and distribution of biological databases and software over low-bandwidth networks among developing countries. J. Bioinformatics, 24 (2), 299-301. IF 4.894
  • Wanna, W., Phongdara, A.and Chotigeat, W. (2006). Sequence variation of the first ribosomal internal transcribed spacer of Penaeus merguiensis in Thailand. Exp. Mol. Biol. Ecol. 331, 64-73. IF 1.66
  • Choosawad, D., Leggat, U., Dechsukhum, C., Phongdara, A., Chotigeat, W.* (2005) Anti-tumour Activities of Fucoidan from the Aquatic Plant Utricularia aureaLour Songklanakarin J. Sci. Technol.27(suppl.3):799-807. (IF 0.118)
  • Wongsawang, P., Phongdara, A., Chanumpai, A. Chotigeat, W.* (2005) Detection ofCHH/GIH hormone activity in fractionated extracts from the eyestalk of Banana prawn. Songklanakarin J. Sci. Technol.27(suppl.3):789-798.IF (IF 0.118)
  • Choosawad, D., Phongdara, A., Chotigeat, W.* (2005) Biological activity of fucoidan from leafy bladderwort (Utricularia aurea Lour). Songklanakarin J. Sci. Technol.27(suppl.3): 809-815. (IF 0.118)
  • Phongdara, A., Hualkasin, W., Wanna, W., Chanumpai, A. and Chotigeat, W. (2004) Species identification of white shrimps Litopenaeus vannamei and other Penaeus sp. by using PCR-RFLP of cytochrome oxidase subunit I (COI) gene.
    Songklanakarin J. Sci. Technol. 26, 4, 467-478. IF 0.091
  • Hualkasin, W., Sirimontaporn, P., Chotigeat, W., Querci, J.and Phongdara, A. (2003). Molecular Phylogenetic Analysis of White Prawns Species and the Existence of Two Clades in Penaeus merguiensis. J. Exp. Mol. Biol. Ecol. 296/1, 1-11. IF 1.66
  • Chotigeat, W.*, Buatong, W., and Phongdara, A. (2001). Detection of Dengue Virus by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) in Serum of Patients. Songklanakarin J. Sci. Technol. 23, 376-381. IF 0.091
  • Chotigeat, W.*, Chayanunnukul, W. and Phongdara, A. (2000). Expression of a Mammalian a 2, 6 sialyltransferase in Pichia pastoris. J. Biotech.81, 65 – 71. IFr 2.687