.

+66-7428-8797 mbb.sci.psu@gmail.com

Asst.Prof.Dr. Warapond Wanna

l-warapond

Present Status: Assistant Professor and Researcher
Address: Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla, Hat Yai, Songkhla, 90112, Thailand.
Telephone: 66-74-288789
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. / This email address is being protected from spambots. You need JavaScript enabled to view it.
Research of Interest: The mechanism of programmed cell death in shrimp, The immune factor responsible for shrimp resistance against viral pathogen

 

Educational Backgrounds

1999 - 2004 Ph.D. (Biochemistry), Faculty of Science, Prince of Songkla University, Songkhla, Thailand
Thesis Title “Development and Application of Nuclear DNA Markers in Penaeus merguiensis”.
1995 - 1999 B.Sc. (Biotechnology, 2nd honors) Faculty of Science, Prince of Songkla University, Songkhla, Thailand.

 

Research Experiences

2009 - present Working on shrimp genome and gene function for diseases protection
2007 - 2009 Working on rainbow trout genome, Division of Animal and Veterinary Sciences, West Virginia University, Morgantown, WV, USA. 
       - Animal cell tissue culture
       - Immunohistrochemistry
       - Microarray
2004 - 2007 Working on shrimp genome and gene function for diseases protection
1999 - 2004 Development and Application of Nuclear DNA Markers in Penaeus merguiensis
1998 - 1999 Cloning of chitinase gene from yeast Saccharomyces cerevisiae

 

Academic Work Experiences

2007 - 2009 Post-doctoral fellow, Laboratory of Animal Biotechnology/Genomics,
Division of Animal and Veterinary Sciences, West Virginia University, Morgantown, WV, USA.
2001 - 2002 Ph.D. student, Génome, Populations, Interactions, Adaptation,
Université Montpellier 2, IFREMER, CNRS UMR 5171, SMEL, 1 Quai de la dadaurade, Sète, France.

 

Scholarships

2007 - 2009 Post-doctoral fellow scholarship, Laboratory of Animal Biotechnology/Genomics,
Division of Animal and Veterinary Sciences, West Virginia University, Morgantown, WV, USA
2001 - 2002 The French Embassy in Thailand.
1999 - 2004 The Royal Golden Jubilee Graduate Program from the Thailand Research Fund (PHD/0085/2542).
1999 - 2004 Graduate School of Prince of Songkla University.
1999 - 2000 The grant number BT-B-06-2B-18-308 from the National Science and Technology Development Agency (NSTDA), Thailand.

 

Awards and Honors

  •  Outstanding doctoral thesis award (2006) in Agriculture and Biology from The National Research Council of Thailand (NRCT).
  •  Component project award for undergraduate student (1998) in biotechnology, Faculty of science, Prince of Songkla University.

Teaching Experiences

  • M.Sc. and Ph.D. Course Molecular Biology, Techniques in genomics and proteomics, Bioinformatic.
  • B.Sc. Course Principle of biotechnology, Marine biotechnology, Genetic engineering, Protein Engineering,

Research Skills

DNA and RNA extraction, Cloning, cDNA library preparation, yeast two hybrid system, RT-PCR, real time PCR, gene expression, protein purification, Southern and Western blotting, Immunohistrochemistry, RNAi and other advanced techniques.

Computer Skills

Microsoft office and bioinformatics tool such as ClustalX/W, GeneDoc, Vector NTI, Rasmol, Protein sequence analysis, Phylip, Gentix and etc.

Current Research Interests

  • The mechanism of programmed cell death in shrimp.
  • The immune factor responsible for shrimp resistance against viral pathogen.
  • DNA Markers for population genetic and species identification.
  • Rainbow trout Genome.

Publications

  • Douangboubpha, B., Bumrungsri, S., Satasook, C., Wanna, W., Soisook, P., Bates, P.J.J. (2016). Morphology, genetics and echolocation calls of the genus Kerivoula (Chiroptera: Vespertilionidae: Kerivoulinae) in Thailand. Mammalia, 80(1), 21-47.
  • Kositanont, U., Wanna, W., Duangchawee, G., and Tribuddharat, C. (2014) Characterization of recombinant flagellin B protein from Leptospira interrogans, Southeast Asian J Trop Med Public Health 45, 1080-1089.
  • Kowasupat, C., Panijpan, B., Laosinchai, P., Ruenwongsa, P., Phongdara, A., Wanna, W., W., Saengchan, S., Phiwsaiya, K. (2014). Biodiversity of the Betta smaragdina (Teleostei: Perciformes) in the northeast region of Thailand as determined by mitochondrial COI and nuclear ITS1 gene sequences. Meta Gene 2(0): 83-95.
  • Panijpan, B., Kowasupat, C., Laosinchai, P., Ruenwongsa, P., Phongdara, A., Senapin, S., Wanna, W., Phiwsaiya, K., Kühne, J.,Fasquel, F. (2014). Southeast Asian mouth-brooding Betta fighting fish (Teleostei: Perciformes) species and their phylogenetic relationships based on mitochondrial COI and nuclear ITS1 DNA sequences and analyses. Meta Gene, 2, 862-879.
  • Wang, L., Tripurani, S. K., Wanna, W., Rexroad, C. E., 3rd, and Yao, J. (2013) Cloning and characterization of a novel oocyte-specific gene encoding an F-Box protein in rainbow trout (Oncorhynchus mykiss), Reprod Biol Endocrinol 11, 86.
  • Panrat, T., Sinthujaroen, P., Nupan, B., Wanna, W., Tammi, M. T., and Phongdara, A. (2012) Characterization of a novel binding protein for Fortilin/TCTP--component of a defense mechanism against viral infection in Penaeus monodon, PLoS One 7, e33291.
  • Wanna, W., Thipwong, J., Mahakaew, W., and Phongdara, A. (2012) Identification and expression analysis of two splice variants of the 14-3-3 epsilon from Litopenaeus Vannamei during WSSV infections, Mol Biol Rep 39, 5487-5493.
  • Saelee, N., Tonganunt-Srithaworn, M., Wanna, W., and Phongdara, A. (2011) Receptor for Activated C Kinase-1 protein from Penaeus monodon (Pm-RACK1) participates in the shrimp antioxidant response, Int J Biol Macromol 49, 32-36.
  • Kongton, K., Phongdara, A., Tonganunt-Srithaworn., M., Wanna, W. (2011). Molecular cloning and expression analysis of the interferonc-γ inducible lysosomal thiol reductase gene from the shrimp Penaeus monodon. Molecular Biology Reports, 38 (5), 3463-3470.
  • Kongton, K., Phongdara, A., Tonganunt-Srithaworn, M., and Wanna, W. (2011) Molecular cloning and expression analysis of the interferon-gamma-inducible lysosomal thiol reductase gene from the shrimp Penaeus monodon, Mol Biol Rep 38, 3463-3470.
  • Graidist, P., Phusantisampan, T., Wanlem, S., Wanna, W. (2010). Detection of pinX1 and 14-3-3 in the shrimp (litopenaeus vannamei) and study on gene expressions during viral infection and environmental stresses. Songklanakarin Journal of Science and Technology, 32(6), 571-579.
  • Wanna, W., & Rexroad, C.E., and Yao, J. Identification of a Functional Splice Variant of 14-3-3E1 in Rainbow Trout. Mar Biotechnol., publish online July 10, 2009 (DOI 10.1007/s10126-009-9201-6)
  • Tonganunt, M., Nupan, B., Saengsakda, M., Suklour, S., Wanna, W., Senapin, S., Chotigeat, W. and Phongdara, A. 2008. The Role of Pm-fortilin in protecting shrimp from White Spot Syndrome Virus (WSSV) infection. Fish and Shellfish Immunology, 25: 633-637.
  • Phongdara, A., Laoong-u-thai, Y., Wanna, W. (2007). Cloning of eIF5A from shrimp Penaeus monodon, a highly expressed protein involved in the survival of WSSV-infected shrimp. Aquaculture, 265(1-4), 16-26.
  • Laoong-u-thai, Y., Chotigeat, W., Wanna, W., and Phongdara, A. 2007. Cloning of eIF5A from shrimp Penaeus monodon, ahighly expressed protein involved in the survival WSSV infected shrimp. Aquaculture, 265: 16-26.
  • Wanna, W., Rolland, J.L., Bonhomme, F., and Phongdara, A. 2004. Population genetic structure of Penaeus merguiensis in Thailand based on nuclear DNA variation. J. Exp. Mar. Biol. Ecol., 311: 63-78.
  • Phongdara, A., Wanna, W., and Chotigeat, W. 2006. Molecular cloning and expression of caspase from white shrimp Penaeus merguiensis. Aquaculture, 252: 114-120.
  • Graidist, P., Fujise, K., Wanna, W., Sritunyalucksana, K. and Phongdara, A. 2006. Establishing a role for shrimp fortilin in preventing cell death. Aquaculture, 255: 157-164.
  • Wanna, W., Hualkasin, W., Chotigeat, W. and Phongdara, A. 2006. Sequence variations of the first ribosomal internal transcribed spacer of Penaeus species in Thailand.J. Exp. Mar. Biol. Ecol., 311: 64-73

Conference Proceedings

Oral presentations

  • Wanna, W., Rolland, J.L., Bonhomme, F., and Phongdara, A., 2004, Genetic Structure of Penaeus merguiensis in Thailand peninsula, RGJ-Ph.D. Congress V, 23-25 April, 2004, Chonburi, Thailand.
  • Wanna, W., Rolland, J.L., Bonhomme, F., Chotigeat, W., and Phongdara, A., 2002, Population genetic of Penaeus merguiensis in Thailand using nuclear DNA markers, The Forth National Symposium on Marine Shrimp, 18-19 November, 2002, Chonburi, Thailand.

 Poster presentations

  • Wang, L., Tripurani, S., Wanna, W., Weber, G.M., Rexroad Iii, C.E., and Yao, J., 2009., Cloning and characterization of a novel oocyte-specific gene encoding an F-Box protein in rainbow trout (Oncorhynchus mykiss), Society for the Study of Reproduction Annual Meeting, July 18-22 2009, David L. Lawrence Convention Center, Pittsburgh, PA. USA.
  • Wanna, W., Rexroad, C.E., and Yao, J., 2008, Genetic Variations of 14-3-3E1 Isoform in rainboe trout (Oncorhynchus mykiss), The International Conference on the Status of Plant & Animal Genome Research. January 12 -16 2008, Town & Country Hotel, San Diego, CA, USA.
  • Wanna, W., Chotigeat, W., and Phongdara, A., 2005, Characterisation of caspase gene from white shrimp Penaeus merguiensis. 18-20 October, 2005, Nakhon Ratchasima, Thailand.
  • Hualkasin, W., Wanna, W., Rungsithum, J., Sirimontaporn, P., Chunumpai, A., Chotigeat, W., Querci, J., and Pongdara, A., 2002. Identification of Penaeus merguiensis and Penaeus silasi by mitochondrial DNA and ITS1. The Forth National Symposium on Marine Shrimp, 18-19 November, 2002, Chonburi, Thailand.
  • Wanna, W., Chotigeat, W., and Pongdara, A., 2001. Analysis of microsatellite DNA,{(TAA)n,(CT)n and (GT)n} from Banana Prawn. RGJ-Ph.D. Congress II, 20-22 April, 2001, Chonburi, Thailand. Hualkasin, W.,
  • Wanna, W., Chotigeat, W., and Pongdara, A., 2000. Cloning of microsatellite DNA , (TAA)n and (CT)n from Banana prawn (Penaeus indicus). The Second National Symposium on Marine Shrimp, 23-25 November, 2000, Phuket, Thailand.
  • Wanna, W., Chunumpai, A., Chotigeat, W., and Pongdara, A., 2001. Mitochondrial COI sequence variation in samples of Penaeus merguiensis (poster presentation). The Third National Symposium on Marine Shrimp, 7-10 November, 2001, Bangkok, Thailand.