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+66-7428-8797 mbbpsuofficial

Dr. Alisa Nakkaew

l-alisa

Present position: Lecturer and Researcher
Address: Faculty of Science, Prince of Songkla University (PSU), Hat Yai, Songkhla 90112 Thailand
Fax Number: +66-74 288777
Telephone: +66-74 288794
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. / This email address is being protected from spambots. You need JavaScript enabled to view it. / This email address is being protected from spambots. You need JavaScript enabled to view it.
Research of Interest: Our next direction is to use the expressed sequence tag (EST) library of oil palm to understand regulation of growth, development and defensive response in the palm.

Educational Qualification

2001 B.Sc. (Biotechnology) Prince of Songkla University, Thailand
2004 M.Sc. (Biochemistry) Prince of Songkla University, Thailand
2014 Ph.D. (Molecular Biotechnology and Bioinformatics) Prince of Songkla University

Research Experiences

  • Study of EST from Oil-Palm and Cloning and Characterization of Lipid synthesis and growth related genes in Oil palm.
  • Study of Molecular marker in Oil Palm (Elaeis quineensis Jacq.) such as Microsatellite, RAPD, Nuclear DNA and EPIC-PCR Markers
  • Bioinformatics tools and application
  • Gene transformation via biolistic and Agrobacterium mediated methods in Oil palm.

Research Skills

  • DNA and RNA extraction, Cloning, cDNA library preparation and Southern blotting analysis
  • Bioinformatics tools and application
  • Gene expression analysis by using RT-PCR and real time PCR technique
  • Protein expression and purification, Western blotting and Immuno histrochemistry
  • Plant tissue culture and Gene transformation

Projects

Molecular Marker for High Yield Selection in Oil Palm (Elaeis guineensis)(2006-2007), Funded by Prince of Songkla University. Study of Transgenic oil palm (Elaeis guineensis Jacq.) with a Calmodulin(CaM) Gene by Particle Bombardment (2008-2011), Funded by Prince of Songkla University.

Patents

  • Phongdara, A., Chotigeat, W. Nakkaew, A. and Nualkaew, S. 2013. Expression and its application of EgP450 protein: TH1301004934. September 05.
  • Phongdara, A., Chotigeat, W. Nakkaew, A. and Puechmongkol, T. 2013. Molecular Marker for identifying the hybrid tenera varieties of Elaeis guineensis Jacq: TH1301002801. May 28.

Awards

Bronze Medal Award, The 6th Sci & Tech Initiative and Sustainability Awards 2013 (STISA 6th), Title "Application of P450 for Detoxification of Herbicide", Team name "The CLEANER", by Ms. Sureeporn Nualkaew, Mr.Tanate Panrat, and Ms. Alisa Nakkaew, Advised by Prof. Dr. Amornrat Phongdara and Assoc. Dr. Wilaiwan Chotigeat, organized by: Thai Sci. & Tech. Initiative and Sustainability Awards

Publications

  • Puwaphut, R., Nakkaew, A., Phongdara, A. 2016. Diversity among 3 cultivars (RRIM600, RRIT251, and PB350) of hevea brasiliensis and secondary metabolite production. Songklanakarin J Sci and Technol, 38(1), 15-22.
  • Ruttajorn, K., Nakkaew, A., Panrat, T., Vanichanon, A., Phongdara, A. 2016. Isolation, expression analysis and characterization of EgNDL, a NDR-like protein in oil palm (Elaeis guineensis Jacq.). Songklanakarin J Science Technol, 38(3), 281-287.
  • Nakkaew, A. *, Phongdara, A., Eksomtramage, T. and Chotigeat, W. 2014. Overexpression and application of β-carboxyltransferase (EgaccD) gene in oil palm (Elaeis guineensis Jacq). Songklanakarin J Sci Technol, (In Press).
  • Nakkaew, A. *, Thitichai, N., Nualkaew, S., Chotigeat, W. * and Phongdara, A. 2013. Cloning, characterization and overexpression of a 14-3-3 protein from oil palm (Elaeis guineensis Jacq). Plant Breeding, 132, 701–710.
  • Hualkasin, W., Thongin, W., Petsean, W., Phongdara, A. and Nakkaew, A*. 2013. Molecular cloning and characterization of the late embryogenesis abundant group 4 (EgLEA4) gene from oil palm (Elaeis guineensis Jacq). Songklanakarin J Sci Technol, 35 (3), 275-285.
  • Kanchanapoom, K.,Nakkaew, A., Kanchanapoom, K. and Phongdara, A. 2012. Efficient biolistic transformation and regeneration capacity of EgTCTP transgene in protocorm-like bodies of Phalaenopsis orchid. Not Bot Horti Agrobo, 40(1):58-64.
  • Phongdara, A., Nakkaew, A., Nualkaew, S. 2012. Isolation of bioactive molecule EgP450 from expressed sequence tags library of oil palm (Elaeis guineensis Jacq). Pharmaceutical Biology, 50:120-127.
  • Phongdara, A., Nakkaew, A., Nualkaew, S. 2012. Isolation of the detoxification enzyme EgP450 from an oil palm EST library. Pharmaceutical Biology, 50(1), 120-127.
  • Nakkaew, A., Chotigeat, W. and Phongdara, A. 2010. Molecular cloning and expression of EgTCTP, encoding a calcium binding protein, enhances the growth of callus in oil palm (Elaeis guineensis, Jacq). Songklanakarin J Sci Technol, 32, 561-569.
  • Kanchanapoom, K., Nakkaew, A., Kanchanapoom, K., and Phongdara, A. 2008. Determination of the most efficient target tissue and helium pressure for biolistic transformation of oil palm (Elaeis guineensis Jacq.). Songklanakarin J. Sci. Technol., 30: 153-157.
  • Nakkaew, A., Chotigeat, W. Eksomtramage, T. and Phongdara, A., 2008, Cloning and expression of a plastid-encoded subunit, beta-carboxyltransferase gene (accD) and a nuclear-encoded subunit, biotin carboxylase of acetyl-CoA carboxylase from oil palm (Elaeis guineensis Jacq). Plant Science, 175: 497-504

Proceedings

  • Thongin, W., Nakkaew, A., Wanna, W. and Phongdara, A. 2013. Sequence Analysis of Drought-tolerance (EgLEA4) Gene from Oil Palm (Elaeis guineensis Jacq).Thai J. Genet. S(1): 266-669. Genetics towards ASEAN. TheAmbassador Hotel, July, 17-19 2013, Bangkok, Thailand.
  • Nakkaew, A., Nualkaew, S., Chotigeat, W. and Phongdara, A. 2013. Molecular Characterization of Eg14-3-3 and its enhances lipid accumulation in transgenic calli .International Conference on Life Science & Biological Engineering. Toshi Hotel ,March 15-17, 2013 ,Tokyo, Japan.
  • Panrat, T., Nualkaew, S., Nakkaew, A., Phongdara, A., and Tammi, M.T. "Discovery of Novel Interactions in Elaeis guineensis Jacq. Detoxification System", Proceeding of the international Conference on Bioinformatics (InCoB2012), Centara Grand at Central Plaza Ladprao Bangkok, Thailand, October 3 – 5, 2012
  • Puechmongkol, T., Nakkaew, A., Wanna, W., and Phongdara, A. 2012. Development of nuclear DNA marker to identify the oil palm F1 hybrid. The First ASEAN Plus Three Graduate Research Congress (AGRC). The Empress Hotel, March 1-2, 2012, Chiang Mai, Thailand.
  • Nualkaew, S., Nakkaew, A., and Phongdara, A. 2012. Isolation of the detoxification enzyme EgP450 from an oil palm expressed sequence tag library. The First ASEAN Plus Three Graduate Research Congress (AGRC). The Empress Hotel, March 1-2, 2012, Chiang Mai, Thailand.
  • Chullasara, A., Nakkaew, A., and Phongdara, A. 2012. Characterization of EgIV from oil palm (Elaeis guineensis Jacq.) and its application. The First ASEAN Plus Three Graduate Research Congress (AGRC). The Empress Hotel, March 1-2, 2012, Chiang Mai, Thailand.
  • Petsean, K., Nakkaew, A., and Phongdara, A. 2012. Cloning of transcription factor gene encoding from oil palm (Elaeis guineensis Jacq.). The First ASEAN Plus Three Graduate Research Congress (AGRC). The Empress Hotel, March 1-2, 2012, Chiang Mai, Thailand.
  • Nakkeaw, A., Chotigeat, W., and Phongdara, A. 2010.Isolation and characterization of the multiple subunit ACCase genes in oil palm. Commission on Higher Education Congress III: University Staff Development Consortium (CHE-USDC Congress III)9-11 September, 2010. Dusit Thani Pattaya. Hotel, Pattaya, Thailand.
  • Ruttajorn, K., Puechmongkol, T., Nakkeaw, A., Chotigeat, W., and Phongdara, A. 2010.Identification of oil palm (Elaeis guineensis Jacq.) using specific gene markers. Commission on Higher Education Congress III: University Staff Development Consortium (CHE-USDC Congress III)9-11 September, 2010. Dusit Thani Pattaya. Hotel, Pattaya, Thailand.
  • Azim, N., Nakkaew, A., Phongdara, A. and Hualkasin, W. 2010. Cloning and Sequence Analysis of LEA4, drought Resistant gene, in Oil Palm (Elaeis guineensis Jacq.). The 7Th IMT-GT UNINET and The 3Th Joint International PSU-UNS Conferences. Prince of Songkla University, October 7-8, 2010,Songkhla, Thailand.
  • Rakngam, P., Nakkeaw, A., Chotigeat, W., and Phongdara, A. 2009. Cloning and nucleotide Sequencing of Calmodulin Gene, CaM, from Oil Palm (Elaeis guineensis Jacq.). Genetics for National Energy Crisis. Thammasat University, March 25-27, 2009, Bangkok, Thailand.
  • Thitichai, N., Nakkeaw, A., Chotigeat, W. and Phongdara, A. 2009. Cloning and Characterization of the 14-3-3 Gene from Oil Palm (Elaeis guineensis Jacq.). Proceeding of the 16th National Genetic Research Conference (Genetics for National Energy Crisis). Thammasat University, 25-27 March 2009, Bangkok, Thailand.
  • Nuankaew, S., Nakkeaw, A., Chotigeat, W. and Phongdara, A. 2009. Cloning and Characterization of the p450 Gene from Oil Palm (Elaeis guineensis Jacq.). Proceeding of the 16th National Genetic Research Conference (Genetics for National Energy Crisis). Thammasat University, 25-27 March 2009, Bangkok, Thailand.
  • Nakkaew, A., Kanchanapoom, K., Chotigeat, W. and Phongdara, A., 2007, Cloning and Characterization of Translationally Controlled Tumor Protein Homologue of Elaeis guineensis, Jacq., 7-9 November 2007, The 6th Asian Crop Science Association Conference. Bangkok, Thailand.
  • Meemak, P. and Nakkaew, A., 2007, Classification of Promoter and Non-Promoter Sequences using Local Sequence Features and Support Vector Machine. The 11thAnnual National Symposium on Computational Science and Engineering (ANSCSE11).
  • Kanchanapoom, K., Nakkaew, A., Kanchanapoom, K.and Phongdara, A., 2006, Biolistic Transformation in oil palm (Elaeis guineensis Jacq.) tissue culture, RGJ Seminar Series XLV: Innovation of Agricultural Resources, Thailand.
  • Nakkaew, A., Phongdara, A. and Chotigeat,W., 2003, Cloning Acetyl-CoA Carboxylase a beta Subunit (accD) from Mesocarp of Oil Palm (Elaeis guineensis Jacq.), 20-22 October, 2003, 29th Congress on Science and Technology of Thailand, Khon Kean, Thailand.
  • Likitthkhajornnuwat, K., Nakkaew, A. and Phongdara, A., 2002, Identification of Polymorphic Oil Palm (Elaeis guineensis Jacq.) Using Microsatellite, Nuclear Gene Marker and RAPD, 28th Congress on Science and technology of Thailand, Bangkok, Thailand.
  • Nakkaew, A., Youkongkaew, N., Likitthkhajornnuwat, K., Chotigeat,W., Asawatreratanakul, P., andPhongdara, A., 2001, Cloning of SSR-DNA (CA)n From Oil Palm (Elaeis guineensis), 27th Congress on Science and Technology of Thailand, Songkhla, Thailand.